In his seminar, we will underscore the importance of large-scale protein studies and provide an overview of the proteomics technology. The main part of his talk will be the application of proteomics to address biological questions. He will delve into how proteomics is used to describe protein expression profiles, identify protein interactions, and analyze post-translational modifications, illustrating these concepts with examples from various biomedical studies. Finally, he will introduce the potential of proteomics in clinical research, particularly in the identification of biomarkers in biofluids.