ProMoIJ (Process Motility in ImageJ) is a pack of ImageJ macros to perform semi-automatic motility analysis of microglial processes in 3D from 2-photon time-lapse experiments. It can also be adapted to analyze motility of non-hollow and non-twisted tubular structures such as cellular filaments, dendrites, dendritic spines, growth cone filopodia, cilia, plant roots, and growing stalactites.